1BYF
STRUCTURE OF TC14; A C-TYPE LECTIN FROM THE TUNICATE POLYANDROCARPA MISAKIENSIS
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | ELLIOTT GX-13 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1998-04-15 |
| Detector | MARRESEARCH |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 54.180, 66.450, 85.900 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 35.000 - 2.000 |
| R-factor | 0.206 * |
| Rwork | 0.207 |
| R-free | 0.25000 |
| Structure solution method | SIRAS |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.023 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SHARP |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 52.700 | 2.050 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.068 | 0.185 |
| Total number of observations | 115502 * | |
| Number of reflections | 20632 | |
| <I/σ(I)> | 8.3 | 4 |
| Completeness [%] | 95.9 | 87.9 |
| Redundancy | 6.6 | 5.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7.2 * | 17 * | PROTEIN WAS CRYSTALLIZED FROM 7% PEG 8000, 0.2 M ZN(ACETATE) AND 100 MM NA( CACODYLATE), PH 6.5 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 0.4 (mM) | |
| 2 | 1 | drop | 12.5 (mM) | ||
| 3 | 1 | drop | D-galactose | 2.5 (mM) | |
| 4 | 1 | drop | Tris | 10 (mM) | |
| 5 | 1 | drop | 25 (mM) | ||
| 6 | 1 | reservoir | PEG8000 | 7 (%(w/v)) | |
| 7 | 1 | reservoir | 0.2 (M) | ||
| 8 | 1 | reservoir | sodium cacodylate | 0.1 (M) |
