1BY4
STRUCTURE AND MECHANISM OF THE HOMODIMERIC ASSEMBLY OF THE RXR ON DNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X11 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X11 |
| Temperature [K] | 113 |
| Detector technology | IMAGE PLATE |
| Collection date | 1997-12-14 |
| Detector | MARRESEARCH |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 70.930, 33.650, 101.400 |
| Unit cell angles | 90.00, 89.95, 90.00 |
Refinement procedure
| Resolution | 10.000 - 2.100 |
| R-factor | 0.219 * |
| Rwork | 0.234 |
| R-free | 0.28800 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2nul |
| RMSD bond length | 0.017 * |
| RMSD bond angle | 2.222 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | X-PLOR (3.851) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 6.000 | 2.180 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.081 | 0.419 |
| Number of reflections | 46828 | |
| <I/σ(I)> | 10.39 | |
| Completeness [%] | 85.7 | 83.2 |
| Redundancy | 1.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7.5 | 8 * | 25 MM TRIS BUFFER (PH 7.5),20% PEG3350, 5 MM MGCL2, 400MM NH4CL |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | Tris | 25 (mM) | |
| 2 | 1 | drop | protein | 0.9 (mM) | |
| 3 | 1 | drop | DNA | 0.45 (mM) | |
| 4 | 1 | reservoir | PEG3350 | 18-23 (%) | |
| 5 | 1 | reservoir | Tris | 25 (mM) | |
| 6 | 1 | reservoir | 5 (mM) | ||
| 7 | 1 | reservoir | 400 (mM) |
