1BXW

OUTER MEMBRANE PROTEIN A (OMPA) TRANSMEMBRANE DOMAIN

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	298
Detector technology	AREA DETECTOR
Collection date	1998-01-15
Detector	SIEMENS
Spacegroup name	C 1 2 1
Unit cell lengths	69.180, 77.950, 50.930
Unit cell angles	90.00, 91.52, 90.00

Refinement procedure

Resolution	50.000 - 2.500
Rwork	0.189
R-free	0.23500
Structure solution method	MIRAS
RMSD bond length	0.015
RMSD bond angle	0.030
Data reduction software	XDS
Data scaling software	CCP4 ((SCALA))
Phasing software	SHARP
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.640
High resolution limit [Å]	2.500	2.500
Rmerge	0.028 *	0.110 *
Number of reflections	8328
<I/σ(I)>	16.8	6.6
Completeness [%]	89.0	53
Redundancy	2.1	1.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	5.1 *		drop contained 1:1 mixture of protein and reservoir solution *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10 (mg/ml)
2	1	drop		0.6 (%(w/v))
3	1	reservoir	PEG8000	12 (%(w/v))
4	1	reservoir	MPD	10 (%(v/v))
5	1	reservoir		25 (mM)