1BXV

REDUCED PLASTOCYANIN FROM SYNECHOCOCCUS SP.

Experimental procedure

Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-6A
Synchrotron site	Photon Factory
Beamline	BL-6A
Temperature [K]	300
Detector technology	DIFFRACTOMETER
Collection date	1997-12
Detector	WEISSENBERG
Spacegroup name	P 41
Unit cell lengths	43.060, 43.060, 57.040
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	10.000 - 1.800
R-factor	0.159
Rwork	0.162
R-free	0.19500
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	OXIDIZED FORM
RMSD bond length	0.016 *
RMSD bond angle	2.400 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	1.860
High resolution limit [Å]	1.800	1.800
Rmerge	0.061	0.203 *
Total number of observations	43919 *
Number of reflections	9373 *
Completeness [%]	96.2	77.1 *
Redundancy	3.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	unknown *	5		pH 5.0

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	protein	30-60 (mg/ml)
2	1	2	ammonium sulfate	1.8-2.0 (M)