1BXU

OXIDIZED PLASTOCYANIN FROM SYNECHOCOCCUS SP.

Experimental procedure

Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-6A
Synchrotron site	Photon Factory
Beamline	BL-6A
Temperature [K]	300
Detector technology	DIFFRACTOMETER
Collection date	1997-12
Detector	WEISSENBERG
Spacegroup name	P 41
Unit cell lengths	43.100, 43.100, 56.900
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	10.000 - 1.900
R-factor	0.151
Rwork	0.167
R-free	0.18700
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1iuz
RMSD bond length	0.016
RMSD bond angle	2.500 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	X-PLOR
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	1.970
High resolution limit [Å]	1.900	1.900
Rmerge	0.071	0.123
Total number of observations	39058 *
Number of reflections	8058
Completeness [%]	99.1	97.3
Redundancy	4.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	unknown *	5.3		pH 5.3

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	protein	30-60 (mg/ml)
2	1	2	ammonium sulfate	1.8-2.0 (M)