1BXN

THE CRYSTAL STRUCTURE OF RUBISCO FROM ALCALIGENES EUTROPHUS TO 2.7 ANGSTROMS.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE BM1A
Synchrotron site	ESRF
Beamline	BM1A
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	1997-05-11
Detector	MARRESEARCH
Spacegroup name	P 43 21 2
Unit cell lengths	112.000, 112.000, 402.700
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	15.000 - 2.700
R-factor	0.266
Rwork	0.266
R-free	0.32200
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1aus
RMSD bond length	0.010
RMSD bond angle	22.760 *
Data scaling software	CCP4
Phasing software	AMoRE
Refinement software	X-PLOR (3.8)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	25.000
High resolution limit [Å]	2.700	2.650 *
Rmerge	0.125	0.695 *
Number of reflections	65785	9546 *
<I/σ(I)>	1.5
Completeness [%]	87.5 *	89.1 *
Redundancy	4.4	4.3 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, sitting drop *	7.5 *	4 *	pH 8.40

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	40 (mg/ml)
2	1	drop	HEPES	20 (mM)
3	1	reservoir		0.7-0.8 (M)