1BXK

DTDP-GLUCOSE 4,6-DEHYDRATASE FROM E. COLI

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	270
Detector technology	AREA DETECTOR
Collection date	1997-10-15
Detector	BRUKER
Spacegroup name	P 21 21 21
Unit cell lengths	68.400, 109.200, 128.700
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.000 - 1.900
Rwork	0.195
Structure solution method	MIR
RMSD bond length	0.014
RMSD bond angle	2.338
Data reduction software	XDS
Data scaling software	XSCALIBRE
Refinement software	TNT (5E)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	1.990
High resolution limit [Å]	1.900	1.900
Rmerge	0.068	0.260
Number of reflections	73785
<I/σ(I)>	12.7	1.6
Completeness [%]	96.0	86
Redundancy	5.3	2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		6		15% PEG 8000 100 MM K/MES PH 6.0