1BXI

CRYSTAL STRUCTURE OF THE ESCHERICHIA COLI COLICIN E9 DNASE DOMAIN WITH ITS COGNATE IMMUNITY PROTEIN IM9

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	EMBL/DESY, HAMBURG BEAMLINE X31
Synchrotron site	EMBL/DESY, HAMBURG
Beamline	X31
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	1996-11-15
Detector	MARRESEARCH
Wavelength(s)	0.979,0.9795,0.9
Spacegroup name	P 21 21 21
Unit cell lengths	43.850, 51.960, 87.590
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	8.000 - 2.050
Rwork	0.218
R-free	0.31500
Structure solution method	MAD
RMSD bond length	0.020
RMSD bond angle	0.046
Data reduction software	DENZO
Data scaling software	SCALEPACK
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.150
High resolution limit [Å]	2.050	2.050
Number of reflections	13096
<I/σ(I)>	22.8	6.7
Completeness [%]	99.6	99.9
Redundancy	8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		5.3		24%(W/V)PEG 4K, 0.1MM SODIUM-ACETATE, PH5.3