1BXC

XYLOSE ISOMERASE FROM THERMUS CALDOPHILUS

Experimental procedure

Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-6A
Synchrotron site	Photon Factory
Beamline	BL-6A
Temperature [K]	287
Detector technology	IMAGE PLATE
Detector	FUJI
Spacegroup name	P 21 21 21
Unit cell lengths	84.350, 123.600, 140.240
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	8.000 - 2.300
R-factor	0.178 *
Rwork	0.178
R-free	0.26500 *
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5xin
RMSD bond length	0.009 *
RMSD bond angle	24.650 *
Data reduction software	WEIS
Data scaling software	CCP4
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall
Low resolution limit [Å]	8.000
High resolution limit [Å]	2.300
Rmerge	0.100
Number of reflections	68336
Completeness [%]	76.0
Redundancy	3.05

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	7.2 *		pH 5.95

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10 (mg/ml)
2	1	drop	sodium phosphate	50 (mM)