1BXB

XYLOSE ISOMERASE FROM THERMUS THERMOPHILUS

Experimental procedure

Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-6A
Synchrotron site	Photon Factory
Beamline	BL-6A
Temperature [K]	287
Detector technology	IMAGE PLATE
Detector	FUJI
Spacegroup name	P 21 21 21
Unit cell lengths	73.340, 144.050, 155.070
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	8.000 - 2.200
R-factor	0.185 *
Rwork	0.180
R-free	0.23200 *
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	23.850 *
Data reduction software	DENZO
Data scaling software	CCP4
Phasing software	X-PLOR (3.843)
Refinement software	X-PLOR (3.843)

Data quality characteristics

	Overall
Low resolution limit [Å]	8.000
High resolution limit [Å]	2.200
Rmerge	0.078
Number of reflections	66746
Completeness [%]	78.0
Redundancy	4.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	7.2 *		pH 8.8

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10 (mg/ml)
2	1	drop	sodium phosphate	50 (mM)