1BWZ
DIAMINOPIMELATE EPIMERASE FROM HEMOPHILUS INFLUENZAE
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU |
Temperature [K] | 288 |
Detector technology | AREA DETECTOR |
Detector | SIEMENS |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 102.100, 115.400, 66.300 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 23.790 - 2.720 |
R-factor | 0.19 |
Rwork | 0.190 |
R-free | 0.24200 |
Structure solution method | MIR |
RMSD bond length | 0.004 |
RMSD bond angle | 28.590 * |
Data reduction software | X-GEN |
Data scaling software | X-GEN |
Refinement software | X-PLOR (3.851) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 23.790 | 2.830 |
High resolution limit [Å] | 2.720 | 2.720 |
Rmerge | 0.060 * | 0.145 * |
Total number of observations | 33313 * | |
Number of reflections | 9691 | |
<I/σ(I)> | 11.61 | 10.89 |
Completeness [%] | 89.4 | 51.6 |
Redundancy | 3.4 | 1.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 7.5 * | 15 * | pH 6.5 |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 20 (mg/ml) | |
2 | 1 | drop | HEPES | 25 (mM) | pH7.5 |
3 | 1 | drop | 0.05 (%) | ||
4 | 1 | reservoir | sodium acetate | 1 (M) | |
5 | 1 | reservoir | sodium cacodylate | 0.1 (M) | pH6.8 |