1BWQ

PROBING THE SUBSTRATE SPECIFICITY OF THE INTRACELLULAR BRAIN PLATELET-ACTIVATING FACTOR ACETYLHYDROLASE

Experimental procedure

Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	1998-01
Detector	RIGAKU
Spacegroup name	P 31 2 1
Unit cell lengths	81.400, 81.400, 72.690
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	8.000 - 2.300
R-factor	0.199 *
Rwork	0.199
R-free	0.27400
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	PAF ACETYLHYDROLASE
RMSD bond length	0.010
RMSD bond angle	0.031
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.490
High resolution limit [Å]	2.300	2.400
Rmerge	0.041 *	0.161 *
Total number of observations	127387 *
Number of reflections	11853
<I/σ(I)>	8.1
Completeness [%]	93.3	87.6 *
Redundancy	54

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, sitting drop *	6.7		pH 6.7

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	5-9 (mg/ml)
2	1	reservoir	ammonium sulfate	13 (%)
3	1	reservoir	Tris-HCl