1BWO

THE CRYSTAL STRUCTURE OF WHEAT NON-SPECIFIC TRANSFER PROTEIN COMPLEXED WITH TWO MOLECULES OF PHOSPHOLIPID AT 2.1 A RESOLUTION

Experimental procedure

Temperature [K]	293
Collection date	1997-05
Spacegroup name	P 21 21 21
Unit cell lengths	41.110, 56.890, 70.630
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	10.000 - 2.100
R-factor	0.163
Rwork	0.163
R-free	0.21300
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.005
RMSD bond angle	23.500 *
Data reduction software	XDS
Data scaling software	XDS
Phasing software	AMoRE
Refinement software	X-PLOR (3.841)

Data quality characteristics

	Overall
Low resolution limit [Å]	24.300 *
High resolution limit [Å]	2.100 *
Rmerge	0.048 *
Total number of observations	23492 *
Number of reflections	9208
<I/σ(I)>	12.6
Completeness [%]	90.5 *
Redundancy	2.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	5.6	20 *	pH 5.6

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	6 (mg/ml)
2	1	reservoir	PEG4000	30 (%(w/v))
3	1	reservoir	ammonium acetate	0.2 (M)
4	1	reservoir	citrate	0.1 (M)