1BWJ

THE 1.8 A STRUCTURE OF MICROGRAVITY GROWN TETRAGONAL HEN EGG WHITE LYSOZYME

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	293
Detector technology	IMAGE PLATE
Detector	RIGAKU RAXIS IIC
Spacegroup name	P 43 21 2
Unit cell lengths	79.245, 79.245, 38.021
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 1.800
R-factor	0.173
Rwork	0.173
R-free	0.21300
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	193l
RMSD bond length	0.009
RMSD bond angle	22.340 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Refinement software	X-PLOR (3.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	99.000
High resolution limit [Å]	1.700	1.700 *
Rmerge	0.072	0.393 *
Total number of observations	57479 *
Number of reflections	11846
<I/σ(I)>	19.8
Completeness [%]	85.5	45.1 *
Redundancy	4.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	liquid-liquid dialysis *	4.7	293	microgravity *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	lysozyme		21mg
2	1	1	acetate	0.04 (M)	0.250ml
3	1	2		1.35 (M)
4	1	2	acetate	0.04 (M)