1BWI
THE 1.8 A STRUCTURE OF MICROBATCH OIL DROP GROWN TETRAGONAL HEN EGG WHITE LYSOZYME
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RU200 |
Temperature [K] | 293 |
Detector technology | IMAGE PLATE |
Detector | RIGAKU RAXIS IIC |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 79.230, 79.230, 37.940 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.000 - 1.800 |
R-factor | 0.171 |
Rwork | 0.171 |
R-free | 0.20600 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 193l |
RMSD bond length | 0.007 |
RMSD bond angle | 22.200 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Refinement software | X-PLOR (3.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 99.000 | |
High resolution limit [Å] | 1.700 | 1.700 * |
Rmerge | 0.077 | 0.151 * |
Total number of observations | 73591 * | |
Number of reflections | 12081 | |
<I/σ(I)> | 22.6 | |
Completeness [%] | 87.2 * | 43.2 * |
Redundancy | 6.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | microbatch method * | 4.7 | 291 | A SOLUTION CONTAINING 40 MG/ML OF LYSOZYME (SIGMA L-6876, BATCH NUMBER, LOT 65H7025, IE A DIFFERENT SIGMA SAMPLE TO THE EXPERIMENTS DESCRIBED ABOVE) IN 10 MM SODIUM CITRATE BUFFER PH 4.7 WAS MIXED IN AN EPPENDORF TUBE WITH EQUAL VOLUMES OF 12 % SODIUM CHLORIDE (BDH). THE MIXTURE WAS FILTERED THROUGH A 0.22 UM FILTER AND 8 UL DROPS WERE MANUALLY DISPENSED UNDER PARAFFIN OIL USING A GILSON MICRO PIPPETTE. OVER 20 IDENTICAL DROPS WERE SET UP AND, WITHIN A WEEK, SINGLE CRYSTALS TYPICALLY MEASURING 0.5 X 0.4 X 0.3 MM WERE OBTAINED IN MOST OF THE DROPS. CRYSTALLISATION TOOK PLACE AT A TEMPERATURE OF 18 - 20 C., drops under paraffin oil, temperature 291K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | 1 | lysozyme | 40 (mg/ml) | |
2 | 1 | 1 | sodium citrate | 10 (mM) | |
3 | 1 | 2 | 12 (%) |