1BWH

THE 1.8 A STRUCTURE OF GROUND CONTROL GROWN TETRAGONAL HEN EGG WHITE LYSOZYME

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	293
Detector technology	IMAGE PLATE
Detector	RIGAKU RAXIS IIC
Spacegroup name	P 43 21 2
Unit cell lengths	79.266, 79.266, 37.956
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 1.800
R-factor	0.184
Rwork	0.184
R-free	0.23300
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	193l
RMSD bond length	0.007
RMSD bond angle	21.920 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Refinement software	X-PLOR (3.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	99.000
High resolution limit [Å]	1.700	1.700 *
Rmerge	0.069	0.344 *
Total number of observations	72955 *
Number of reflections	12309
<I/σ(I)>	22
Completeness [%]	88.8	56.1 *
Redundancy	5.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	liquid-liquid dialysis *	4.7		ground control *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	lysozyme		21mg
2	1	1	acetate	0.04 (M)	0.250ml
3	1	2		1.35 (M)
4	1	2	acetate	0.04 (M)