1BW0
CRYSTAL STRUCTURE OF TYROSINE AMINOTRANSFERASE FROM TRYPANOSOMA CRUZI
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | SIEMENS |
Temperature [K] | 285 |
Detector technology | AREA DETECTOR |
Collection date | 1996-06-15 |
Detector | SIEMENS X1000 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 61.600, 102.200, 77.900 |
Unit cell angles | 90.00, 110.30, 90.00 |
Refinement procedure
Resolution | 10.000 - 2.500 |
R-factor | 0.157 * |
Rwork | 0.157 |
R-free | 0.21400 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1art |
RMSD bond length | 0.006 |
RMSD bond angle | 0.023 |
Data reduction software | X-GEN |
Data scaling software | CCP4 ((SCALA)) |
Phasing software | AMoRE |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 10.000 | 2.640 |
High resolution limit [Å] | 2.500 | 2.500 |
Rmerge | 0.051 | 0.096 |
Number of reflections | 24668 | |
<I/σ(I)> | 17 | 6 |
Completeness [%] | 78.4 | 47 |
Redundancy | 1.7 | 1.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Microdialysis * | 7 | 285 * | DIALYSIS OF 2.6 MG/ML PROTEIN AGAINST 25 % (W/W) PEG 8000, 5 MM PLP, 0.1 M PHOSPHATE/CITRATE, PH 7.0, 285 K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | 1 | PEG8000 | 25 (%(w/w)) | |
2 | 1 | 1 | PLP | 5 (mM) | |
3 | 1 | 1 | citrate/phosphate | 0.1 (M) |