1BVZ

ALPHA-AMYLASE II (TVAII) FROM THERMOACTINOMYCES VULGARIS R-47

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU300
Temperature [K]	113
Detector technology	IMAGE PLATE
Collection date	1998-04-15
Detector	RIGAKU
Spacegroup name	P 21 21 21
Unit cell lengths	114.600, 117.900, 114.000
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	7.000 - 2.600
R-factor	0.196
Rwork	0.196
R-free	0.27200
Structure solution method	MIRAS
RMSD bond length	0.012
RMSD bond angle	2.800
Data scaling software	CCP4
Phasing software	MLPHARE
Refinement software	X-PLOR (3.851)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.730
High resolution limit [Å]	2.600	2.600
Rmerge	0.079	0.286
Total number of observations	91759 *
Number of reflections	43840
<I/σ(I)>	5.31	1.9
Completeness [%]	91.8	78.1
Redundancy	2.1	1.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	6.1	11 *	pH 6.1

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	PEG6000	2 (%(w/v))
2	1	reservoir		2.5 (mM)
3	1	reservoir	MES	20 (mM)	pH6.1
4	1	drop	protein	20 (mg/ml)