1BVW
CELLOBIOHYDROLASE II (CEL6A) FROM HUMICOLA INSOLENS
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SRS BEAMLINE PX9.6 |
Synchrotron site | SRS |
Beamline | PX9.6 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 1997-12-15 |
Detector | MARRESEARCH |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 47.510, 68.100, 53.710 |
Unit cell angles | 90.00, 110.93, 90.00 |
Refinement procedure
Resolution | 15.000 - 1.920 |
R-factor | 0.14 * |
Rwork | 0.142 |
R-free | 0.21000 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1cb2 |
RMSD bond length | 0.008 |
RMSD bond angle | 0.023 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 1.990 |
High resolution limit [Å] | 1.920 | 1.920 |
Rmerge | 0.063 | 0.187 |
Number of reflections | 90583 | |
<I/σ(I)> | 20 | 7.3 |
Completeness [%] | 99.7 | 99.1 |
Redundancy | 3.8 | 3.83 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 7 | PROTEIN WAS CONCENTRATED TO 20 MG/ML IN WATER. CRYSTALLISATION IN 200MM MAGNESIUM ACETATE IN 100MM TRIETHANOLAMINE BUFFER AT PH 7.0. PRECIPITANT WAS 22%. POLYETHYLENE GLYCOL 8000. |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 20 (mg/ml) | |
2 | 1 | reservoir | PEG8000 | 22 (%) | |
3 | 1 | reservoir | magnesium acetate | 200 (mM) | |
4 | 1 | reservoir | triethanolamine | 100 (mM) |