1BVW
CELLOBIOHYDROLASE II (CEL6A) FROM HUMICOLA INSOLENS
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX9.6 |
| Synchrotron site | SRS |
| Beamline | PX9.6 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1997-12-15 |
| Detector | MARRESEARCH |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 47.510, 68.100, 53.710 |
| Unit cell angles | 90.00, 110.93, 90.00 |
Refinement procedure
| Resolution | 15.000 - 1.920 |
| R-factor | 0.14 * |
| Rwork | 0.142 |
| R-free | 0.21000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1cb2 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.023 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.990 |
| High resolution limit [Å] | 1.920 | 1.920 |
| Rmerge | 0.063 | 0.187 |
| Number of reflections | 90583 | |
| <I/σ(I)> | 20 | 7.3 |
| Completeness [%] | 99.7 | 99.1 |
| Redundancy | 3.8 | 3.83 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7 | PROTEIN WAS CONCENTRATED TO 20 MG/ML IN WATER. CRYSTALLISATION IN 200MM MAGNESIUM ACETATE IN 100MM TRIETHANOLAMINE BUFFER AT PH 7.0. PRECIPITANT WAS 22%. POLYETHYLENE GLYCOL 8000. |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 20 (mg/ml) | |
| 2 | 1 | reservoir | PEG8000 | 22 (%) | |
| 3 | 1 | reservoir | magnesium acetate | 200 (mM) | |
| 4 | 1 | reservoir | triethanolamine | 100 (mM) |
