1BVI
RIBONUCLEASE T1 (WILDTYPE) COMPLEXED WITH 2'GMP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 293 |
| Detector technology | IMAGE PLATE |
| Collection date | 1997-12-15 |
| Detector | MARRESEARCH |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 59.340, 60.590, 100.930 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.900 |
| R-factor | 0.177 |
| Rwork | 0.177 |
| R-free | 0.21900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1rga |
| RMSD bond length | 0.006 |
| RMSD bond angle | 24.650 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR (3.851) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.990 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.061 * | |
| Total number of observations | 138761 * | |
| Number of reflections | 28421 | |
| <I/σ(I)> | 15.86 | 4.75 |
| Completeness [%] | 96.7 | 77.1 |
| Redundancy | 4.88 | 3.26 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | unknown * | 4.2 | 25 MM NAAC PH 4.2 6.25 MM CACL2 47.5 % MPD |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | 1 | protein | 10 (mg/ml) | |
| 2 | 1 | 1 | 25 (mM) | ||
| 3 | 1 | 1 | 2'GMP | 2.8 (mM) | |
| 4 | 1 | 1 | 6.25 (mM) | ||
| 5 | 1 | 1 | MPD | 47.5 (%) |
