1BV1
BIRCH POLLEN ALLERGEN BET V 1
Experimental procedure
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH2R |
| Temperature [K] | 287 |
| Detector technology | IMAGE PLATE |
| Collection date | 1996-01 |
| Detector | RIGAKU |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 32.160, 74.240, 118.120 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.000 - 2.000 |
| R-factor | 0.198 |
| Rwork | 0.198 |
| R-free | 0.26700 |
| Structure solution method | MIR |
| RMSD bond length | 0.006 |
| RMSD bond angle | 25.700 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | X-PLOR (3.851) |
| Refinement software | X-PLOR (3.851) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.030 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.068 | 0.360 |
| Total number of observations | 54458 * | |
| Number of reflections | 9554 | |
| <I/σ(I)> | 14.6 | 2.8 |
| Completeness [%] | 95.8 | 84.2 |
| Redundancy | 4.8 | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, sitting drop * | 6.3 | seeding * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | recombinant Bet v 1 | 2.5 (mg/ml) | |
| 2 | 1 | drop | ammonium sulfate | 1.0 (M) | |
| 3 | 1 | drop | sodium citrate | 50 (mM) | |
| 4 | 1 | drop | sodium azide | 0.005 (%) | |
| 5 | 1 | reservoir | ammonium sulphate | 2.0 (M) | |
| 6 | 1 | reservoir | sodium citrate | 100 (mM) |
