1BUH
CRYSTAL STRUCTURE OF THE HUMAN CDK2 KINASE COMPLEX WITH CELL CYCLE-REGULATORY PROTEIN CKSHS1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | SIEMENS |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 1995-12-15 |
Detector | MARRESEARCH |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 56.800, 69.950, 60.530 |
Unit cell angles | 90.00, 114.50, 90.00 |
Refinement procedure
Resolution | 20.000 - 2.600 |
R-factor | 0.19 |
Rwork | 0.190 |
R-free | 0.25000 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | PERSONAL COMMUNICATION |
RMSD bond length | 0.012 |
RMSD bond angle | 1.600 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | X-PLOR (3.8) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 20.000 |
High resolution limit [Å] | 2.600 |
Rmerge | 0.100 |
Total number of observations | 84909 * |
Number of reflections | 13023 * |
Completeness [%] | 97.0 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion * | 7.5 | 20 * | 17-19% MEPEG 5K, 100 MM TRIS-HCL PH 7.5, 100 MM KCL |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 15-20 (mg/ml) | |
2 | 1 | reservoir | MEPEG5000 | 17-19 (%) | |
3 | 1 | reservoir | Tris | 100 (mM) | |
4 | 1 | reservoir | 100 (mM) |