1BUC

THREE-DIMENSIONAL STRUCTURE OF BUTYRYL-COA DEHYDROGENASE FROM MEGASPHAERA ELSDENII

Experimental procedure

Spacegroup name	P 4 2 2
Unit cell lengths	107.800, 107.800, 153.700
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	8.000 - 2.500
R-factor	0.193
Rwork	0.193
RMSD bond length	0.010
RMSD bond angle	1.400
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall
High resolution limit [Å]	2.500 *
Rmerge	0.080 *
Total number of observations	81262 *
Number of reflections	25601
Completeness [%]	80.0

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	6 *	19 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	20 (mg/ml)
2	1	drop	potassium phosphate	120 (mM)
3	1	drop	Bis-Tris acetate	60 (mM)
4	1	drop	ammonium sulfate	190 (mM)
5	1	drop	PEG4500	4.3 (%)
6	1	reservoir	PEG4500	50 (%)