1BU4
RIBONUCLEASE 1 COMPLEX WITH 2'GMP
Experimental procedure
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH2R |
| Temperature [K] | 293 |
| Detector technology | IMAGE PLATE |
| Collection date | 1998-02 |
| Detector | MARRESEARCH |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 40.510, 48.000, 50.810 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.900 |
| R-factor | 0.18 * |
| Rwork | 0.180 |
| R-free | 0.23000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | |
| RMSD bond length | 0.009 * |
| RMSD bond angle | 2.032 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.950 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.139 * | 0.583 * |
| Total number of observations | 44006 * | |
| Number of reflections | 7853 * | |
| <I/σ(I)> | 12.66 | 2.47 |
| Completeness [%] | 95.6 | 92.2 |
| Redundancy | 5.6 * | 5.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 4.2 * | Zegers, I., (1998) Nat. Struct. Biol., 5, 280. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 15 (mg/ml) | |
| 2 | 1 | drop | 2'GMP | 2 (mM) | |
| 3 | 1 | drop | 20 (mM) | ||
| 4 | 1 | drop | 2 (mM) | ||
| 5 | 1 | reservoir | MPD | 55 (%(v/v)) |
