1BTN
STRUCTURE OF THE BINDING SITE FOR INOSITOL PHOSPHATES IN A PH DOMAIN
Experimental procedure
Temperature [K] | 293 |
Detector technology | IMAGE PLATE |
Collection date | 1995-02 |
Detector | MARRESEARCH |
Spacegroup name | P 4 21 2 |
Unit cell lengths | 69.000, 69.000, 50.800 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 8.000 - 2.000 |
R-factor | 0.205 |
Rwork | 0.205 |
R-free | 0.28600 |
RMSD bond length | 0.011 |
RMSD bond angle | 25.620 * |
Data reduction software | XDS |
Phasing software | X-PLOR (3.1) |
Refinement software | X-PLOR (3.1) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 1000.000 |
High resolution limit [Å] | 2.000 |
Rmerge | 0.047 |
Total number of observations | 41713 * |
Number of reflections | 7830 |
Completeness [%] | 89.6 |
Redundancy | 5.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 6.5 | pH 6.5 |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 20 (mg/ml) | |
2 | 1 | drop | sodium phosphate | 10 (mM) | |
3 | 1 | drop | Ins(1,4,5)P3 | 3-fold excess | |
4 | 1 | reservoir | PEG6000 | 10-15 (%(w/v)) | |
5 | 1 | reservoir | ethanol | 30 (%(v/v)) | |
6 | 1 | reservoir | sodium acetate | 100 (mM) |