1BTM
TRIOSEPHOSPHATE ISOMERASE (TIM) COMPLEXED WITH 2-PHOSPHOGLYCOLIC ACID
Experimental procedure
Source type | ROTATING ANODE |
Source details | RIGAKU RU200 |
Detector technology | AREA DETECTOR |
Detector | SIEMENS |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 78.610, 108.420, 71.210 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 8.000 * - 2.800 |
R-factor | 0.176 |
Rwork | 0.176 |
RMSD bond length | 0.010 |
RMSD bond angle | 24.300 * |
Data reduction software | XENGEN |
Phasing software | X-PLOR |
Refinement software | X-PLOR |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 3.000 | |
High resolution limit [Å] | 2.800 | 2.800 |
Rmerge | 0.091 | |
Total number of observations | 64784 * | |
Number of reflections | 16996 * | |
Completeness [%] | 89.5 | 76.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 6.5 * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 4 (mg/ml) | |
10 | 1 | reservoir | EDTA | 1 (mM) | |
11 | 1 | reservoir | sodium azide | 1 (mM) | |
2 | 1 | drop | MES | 5 (mM) | |
3 | 1 | drop | EDTA | 1 (mM) | |
4 | 1 | drop | sodium azide | 1 (mM) | |
5 | 1 | drop | 2-phosphoglycolic acid | 2 (mM) | |
6 | 1 | reservoir | PEG4000 | 25 (%(w/v)) | |
7 | 1 | reservoir | acetate | 100 (mM) | |
8 | 1 | reservoir | isopropanol | 8 (%) | |
9 | 1 | reservoir | dithiothreitol | 2 (mM) |