1BTJ
HUMAN SERUM TRANSFERRIN, RECOMBINANT N-TERMINAL LOBE, APO FORM, CRYSTAL FORM 2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 113 |
| Detector technology | IMAGE PLATE |
| Collection date | 1996-05-15 |
| Detector | RIGAKU RAXIS IIC |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 63.000, 77.300, 72.600 |
| Unit cell angles | 90.00, 104.30, 90.00 |
Refinement procedure
| Resolution | 6.000 - 3.200 |
| R-factor | 0.217 |
| Rwork | 0.217 |
| R-free | 0.31200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | HUMAN TRANSFERRIN N-LOBE HOLO FORM INDIVIDUAL DOMAINS |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.900 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 3.500 |
| High resolution limit [Å] | 3.200 | 3.200 |
| Rmerge | 0.111 | 0.257 |
| Number of reflections | 11269 | |
| <I/σ(I)> | 5.9 | 2.6 |
| Completeness [%] | 99.7 | 99.9 |
| Redundancy | 3.3 | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.3 | 4 * | PROTEIN DROP: 20 MG/ML PROTEIN, 20MM NAHCO3, 50 MM KCL. RESERVOIR: 50 MM POTASSIUM ACETATE, PH 5.3, 35% MPD., VAPOR DIFFUSION, SITTING DROP |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 20 (mg/ml) | |
| 2 | 1 | drop | 20 (mM) | ||
| 3 | 1 | drop | 50 (mM) | ||
| 4 | 1 | reservoir | potassium acetate | 50 (mM) | |
| 5 | 1 | reservoir | MPD | 35 (%(v/v)) |
