1BT9
OMPF PORIN MUTANT D74A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Temperature [K] | 276 |
| Detector technology | IMAGE PLATE |
| Detector | MARRESEARCH |
| Spacegroup name | P 3 2 1 |
| Unit cell lengths | 118.500, 118.500, 52.700 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 20.000 - 3.000 |
| R-factor | 0.191 * |
| Rwork | 0.191 |
| R-free | 0.26900 |
| Structure solution method | OTHER |
| RMSD bond length | 0.010 |
| RMSD bond angle | 0.039 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | SFALL |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 20.000 |
| High resolution limit [Å] | 3.000 |
| Rmerge | 0.082 |
| Number of reflections | 8162 |
| Completeness [%] | 92.0 |
| Redundancy | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Microdialysis * | 7 | Pauptit, R.A., (1991) J. Mol. Biol., 218, 505. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | 1 | protein | 10 (mg/ml) | |
| 2 | 1 | 2 | PEG2000 | 10.5 (%) |
