1BT0
STRUCTURE OF UBIQUITIN-LIKE PROTEIN, RUB1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU |
Temperature [K] | 100 |
Detector technology | AREA DETECTOR |
Collection date | 1997-08-15 |
Detector | SIEMENS |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 28.600, 35.600, 37.900 |
Unit cell angles | 90.00, 102.80, 90.00 |
Refinement procedure
Resolution | 30.000 - 1.700 |
R-factor | 0.18 * |
R-free | 0.24600 * |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1UBI WITH NON-CONSERVED RESIDUES TRUNCATED INTO ALANINE |
RMSD bond length | 0.015 |
RMSD bond angle | 16.000 * |
Data reduction software | XDS |
Data scaling software | CCP4 ((AGROVATA) |
Phasing software | AMoRE |
Refinement software | TNT |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 30.000 |
High resolution limit [Å] | 1.700 |
Rmerge | 0.051 |
Total number of observations | 23228 * |
Number of reflections | 7919 |
Completeness [%] | 95.0 |
Redundancy | 2.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Batch method * | 6.5 | pH 6.5 |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | 1 | protein | 9.0 (mg/ml) | |
2 | 1 | 1 | PEG8000 | 18-20 (%(w/v)) | |
3 | 1 | 1 | zinc acetate | 200-400 (mM) | |
4 | 1 | 1 | MPD | 2-4 (%) | |
5 | 1 | 1 | MES | 100 (mM) |