1BSS
ECORV-T93A/DNA/CA2+
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RU200 |
Temperature [K] | 293 |
Detector technology | IMAGE PLATE |
Collection date | 1995-08-01 |
Detector | RIGAKU |
Spacegroup name | P 1 |
Unit cell lengths | 50.000, 64.400, 49.200 |
Unit cell angles | 109.20, 108.70, 95.80 |
Refinement procedure
Resolution | 6.000 - 2.150 |
R-factor | 0.185 * |
Rwork | 0.184 |
R-free | 0.28800 * |
Structure solution method | OTHER |
Starting model (for MR) | PDB 1AZ0 |
RMSD bond length | 0.011 * |
RMSD bond angle | 1.940 * |
Phasing software | X-PLOR |
Refinement software | X-PLOR |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 20.000 |
High resolution limit [Å] | 2.150 |
Rmerge | 0.074 |
Number of reflections | 31018 |
Completeness [%] | 75.0 |
Redundancy | 2.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 7.5 | pH 7.50 |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 13 (mg/ml) | |
2 | 1 | drop | DNA | protein/DNA stoichiometry is 1:1 | |
3 | 1 | reservoir | PEG4000 | 2-8 (%) | |
4 | 1 | reservoir | 2-propanol | 10 (mM) | |
5 | 1 | reservoir | HEPES | 50 (mM) | |
6 | 1 | reservoir | 100 (mM) |