1BSS

ECORV-T93A/DNA/CA2+

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	293
Detector technology	IMAGE PLATE
Collection date	1995-08-01
Detector	RIGAKU
Spacegroup name	P 1
Unit cell lengths	50.000, 64.400, 49.200
Unit cell angles	109.20, 108.70, 95.80

Refinement procedure

Resolution	6.000 - 2.150
R-factor	0.185 *
Rwork	0.184
R-free	0.28800 *
Structure solution method	OTHER
Starting model (for MR)	PDB 1AZ0
RMSD bond length	0.011 *
RMSD bond angle	1.940 *
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall
Low resolution limit [Å]	20.000
High resolution limit [Å]	2.150
Rmerge	0.074
Number of reflections	31018
Completeness [%]	75.0
Redundancy	2.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	7.5		pH 7.50

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	13 (mg/ml)
2	1	drop	DNA		protein/DNA stoichiometry is 1:1
3	1	reservoir	PEG4000	2-8 (%)
4	1	reservoir	2-propanol	10 (mM)
5	1	reservoir	HEPES	50 (mM)
6	1	reservoir		100 (mM)