1BSR
BOVINE SEMINAL RIBONUCLEASE STRUCTURE AT 1.9 ANGSTROMS RESOLUTION
Experimental procedure
Spacegroup name | P 2 21 21 |
Unit cell lengths | 36.500, 66.700, 107.500 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 6.000 * - 1.900 |
R-factor | 0.177 |
Rwork | 0.177 |
RMSD bond length | 0.020 |
RMSD bond angle | 3.700 |
Phasing software | X-PLOR |
Refinement software | X-PLOR |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 9999.000 * |
High resolution limit [Å] | 1.900 * |
Rmerge | 0.067 * |
Total number of observations | 59869 * |
Number of reflections | 17217 * |
Completeness [%] | 90.0 * |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Batch method * | 5.1 * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | 1 | enzyme solution | 15-20 (mg/ml) | |
2 | 1 | 1 | ammonium sulphate | 80 (%sat) | |
3 | 1 | 1 | ammonium phosphate | 0.1 (mM) | |
4 | 1 | 1 | acetate | 10 (mM) |