1BSE
CRYSTAL STRUCTURAL ANALYSIS OF MUTATIONS IN THE HYDROPHOBIC CORES OF BARNASE
Experimental procedure
| Spacegroup name | P 32 |
| Unit cell lengths | 58.910, 58.910, 81.680 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | ? - 2.000 |
| R-factor | 0.178 |
| RMSD bond length | 0.017 |
| RMSD bond angle | 2.600 * |
| Refinement software | PROLSQ |
Data quality characteristics
| Overall | |
| High resolution limit [Å] | 2.000 * |
| Rmerge | 0.100 * |
| Completeness [%] | 91.2 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7.5 * | 23 * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | ammonium sulfate | 45 (%sat) | |
| 2 | 1 | reservoir | ammonium phosphate | 3.0 (M) | |
| 3 | 1 | reservoir | zinc sulphate | 0.02 (M) | |
| 4 | 1 | drop | protein | 10-20 (mg/ml) |
