1BS5
PEPTIDE DEFORMYLASE AS ZN2+ CONTAINING FORM
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | ELLIOTT GX-18 |
Temperature [K] | 293 |
Detector technology | AREA DETECTOR |
Collection date | 1997-07-15 |
Detector | SIEMENS-NICOLET X100 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 143.400, 64.100, 84.600 |
Unit cell angles | 90.00, 123.20, 90.00 |
Refinement procedure
Resolution | 6.000 - 2.500 |
R-factor | 0.208 |
Rwork | 0.208 |
R-free | 0.25800 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1icj |
RMSD bond length | 0.012 |
RMSD bond angle | 1.200 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | X-PLOR (3.851) |
Refinement software | X-PLOR (3.851) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 2.640 |
High resolution limit [Å] | 2.500 | 2.500 |
Rmerge | 0.076 | 0.338 |
Total number of observations | 130772 * | |
Number of reflections | 22399 | |
<I/σ(I)> | 15.72 | 1.9 |
Completeness [%] | 99.3 | 99.4 |
Redundancy | 5.8 | 2.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 7.4 | Groche, D., (1998) Biochem.Biophys.Res.Comm., 246, 342. * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | reservoir | ammonium sulfate | 2 (M) | |
2 | 1 | reservoir | MOPS-NH3 | 0.2 (M) | |
3 | 1 | reservoir | TCEP | 10 (mM) | |
4 | 1 | drop | 30 (mg/ml) | ||
5 | 1 | drop | PEG1000 | 1 (%(w/v)) |