1BS2
YEAST ARGINYL-TRNA SYNTHETASE
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | EMBL/DESY, HAMBURG BEAMLINE BW7B |
Synchrotron site | EMBL/DESY, HAMBURG |
Beamline | BW7B |
Temperature [K] | 120 |
Detector technology | IMAGE PLATE |
Collection date | 1997-06-15 |
Detector | MARRESEARCH |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 100.374, 100.374, 204.341 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.000 - 2.750 |
R-factor | 0.207 |
Rwork | 0.207 |
R-free | 0.26900 |
Structure solution method | MIRAS |
RMSD bond length | 0.006 |
RMSD bond angle | 20.200 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | CCP4 |
Refinement software | CNS (0.1-0.4) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.850 |
High resolution limit [Å] | 2.750 | 2.750 |
Rmerge | 0.042 * | 0.180 * |
Total number of observations | 168917 * | |
Number of reflections | 27890 | 2660 * |
<I/σ(I)> | 35 | 7.3 |
Completeness [%] | 99.4 | 80 * |
Redundancy | 6 | 4.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 7 | 17 * | AMMONIUM SULFATE 2.45 M, 100MM TRIS-HCL, PH 7.0 AT 290K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | reservoir | ammonium sulfate | 2.45 (M) | |
2 | 1 | reservoir | Tris-HCl | 100 (mM) | |
3 | 1 | drop | protein | 13.5 (mg/ml) | |
4 | 1 | drop | L-arginine | 5 (mM) | |
5 | 1 | drop | ATP | 5 (mM) | |
6 | 1 | drop | 10 (mM) | ||
7 | 1 | drop | Tris-HCl | 50 (mM) |