1BRW
THE CRYSTAL STRUCTURE OF PYRIMIDINE NUCLEOSIDE PHOSPHORYLASE IN A CLOSED CONFORMATION
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE A1 |
| Synchrotron site | CHESS |
| Beamline | A1 |
| Temperature [K] | 108 |
| Detector technology | CCD |
| Collection date | 1997-02-15 |
| Detector | ADSC |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 53.570, 70.450, 122.780 |
| Unit cell angles | 90.00, 98.02, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.100 |
| R-factor | 0.232 |
| Rwork | 0.232 |
| R-free | 0.27600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2tpt |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.200 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR (3.8) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | |
| High resolution limit [Å] | 2.000 | 2.000 * |
| Rmerge | 0.067 * | 0.232 * |
| Total number of observations | 396276 * | |
| Number of reflections | 55716 | |
| Completeness [%] | 88.4 | 41.7 * |
| Redundancy | 2.9 | 1.6 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7 * | Zhou, M., (1998) Acta Cryst., D55, 287. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | potassium phosphate | 10 (mM) | |
| 2 | 1 | drop | protein | 10 (mg/ml) | |
| 3 | 1 | reservoir | ammonium sulfate | 15 (%) | |
| 4 | 1 | reservoir | citrate | 50 (mM) | |
| 5 | 1 | reservoir | dithiothreitol | 4 (mM) |
