1BRI

BARNASE MUTANT WITH ILE 76 REPLACED BY ALA

Experimental procedure

Source type	SYNCHROTRON
Source details	SRS
Synchrotron site	SRS
Detector technology	IMAGE PLATE
Detector	MARRESEARCH
Spacegroup name	P 32
Unit cell lengths	59.360, 59.360, 81.600
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	7.000 - 1.900
R-factor	0.16
RMSD bond length	0.013
RMSD bond angle	0.042
Data reduction software	MOSFLM
Refinement software	PROLSQ

Data quality characteristics

	Overall
High resolution limit [Å]	1.900 *
Rmerge	0.080
Total number of observations	35673 *
Number of reflections	20394
Completeness [%]	80.5
Redundancy	1.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *		23 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10-20 (mg/ml)
2	1	reservoir	ammonium sulfate	45 (%sat)
3	1	reservoir	ammonium phosphate	3.0 (M)
4	1	reservoir	zinc sulfate	0.02 (M)