1BRG

CRYSTALLOGRAPHIC ANALYSIS OF PHE->LEU SUBSTITUTION IN THE HYDROPHOBIC CORE OF BARNASE

Experimental procedure

Spacegroup name	P 32
Unit cell lengths	58.723, 58.723, 82.026
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	6.000 - 2.200
R-factor	0.168
Rwork	0.168
RMSD bond length	0.020
Phasing software	X-PLOR
Refinement software	PROLSQ

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	6.000 *
High resolution limit [Å]	2.200 *	2.200 *
Rmerge	0.080 *	0.053 *
Total number of observations	26504 *
Number of reflections	15097 *
Completeness [%]	94.0 *	77.1 *
Redundancy	1.5 *	1.4 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *			Mauguen, Y., (1982) Nature, 297, 162. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	15 (mg/ml)
2	1	reservoir	phosphate	2.8 (M)
3	1	reservoir		2 (mM)