1BRA

RELOCATING A NEGATIVE CHARGE IN THE BINDING POCKET OF TRYPSIN

Experimental procedure

Spacegroup name	I 2 3
Unit cell lengths	124.400, 124.400, 124.400
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	6.000 - 2.200
R-factor	0.156
RMSD bond length	0.012
RMSD bond angle	3.200
Refinement software	PROLSQ

Data quality characteristics

	Overall
High resolution limit [Å]	2.200 *
Rmerge	0.089 *
Total number of observations	50400 *
Number of reflections	18200 *
Completeness [%]	73.0 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	8 *	17 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	trypsin	10 (mg/ml)
2	1	reservoir		25 (%)
3	1	reservoir	Tris-HCl	40 (mM)
4	1	reservoir	benzamidine	75 (mM)