1BR4
SMOOTH MUSCLE MYOSIN MOTOR DOMAIN-ESSENTIAL LIGHT CHAIN COMPLEX WITH MGADP.BEF3 BOUND AT THE ACTIVE SITE
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE A1 |
| Synchrotron site | CHESS |
| Beamline | A1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 1997-07 |
| Spacegroup name | P 1 |
| Unit cell lengths | 95.320, 144.660, 147.290 |
| Unit cell angles | 111.21, 106.10, 92.58 |
Refinement procedure
| Resolution | 10.000 - 3.620 |
| R-factor | 0.277 |
| Rwork | 0.277 |
| R-free | 0.35200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | SMOOTH MUSCLE MOTOR DOMAIN |
| RMSD bond length | 0.009 |
| RMSD bond angle | 27.600 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | X-PLOR (3.851) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 3.900 |
| High resolution limit [Å] | 3.620 | 3.620 |
| Rmerge | 0.111 * | 0.331 * |
| Total number of observations | 478668 * | |
| Number of reflections | 75979 | |
| <I/σ(I)> | 13.7 | 6.9 |
| Completeness [%] | 95.6 | 94.2 |
| Redundancy | 6.3 | 4.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7.2 | 4 * | 1.8M AMMONIUM SULFATE 100MM HEPES PH 7.2 2MM MGADP 2MM AL(NO3)3 8MM NAF PROTEIN CONCENTRATION: 10MG/ML TEMPERATURE: 4 DEGREES CENTIGRADE, temperature 277K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10 (mg/ml) | |
| 2 | 1 | drop | MgADP | 2 (mM) | |
| 3 | 1 | drop | beryllium(SIGMA) | 2 (mM) | |
| 4 | 1 | drop | (mM) | ||
| 5 | 1 | reservoir | ammonium sulfate | (M) | |
| 6 | 1 | reservoir | HEPES | (mM) |
