1BR3

CRYSTAL STRUCTURE OF AN 82-NUCLEOTIDE RNA-DNA COMPLEX FORMED BY THE 10-23 DNA ENZYME

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-1
Synchrotron site	SSRL
Beamline	BL9-1
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	1998-02-15
Spacegroup name	P 61 2 2
Unit cell lengths	63.450, 63.450, 216.510
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	30.000 - 3.000
R-factor	0.227
Rwork	0.227
R-free	0.25100
Structure solution method	MIR
RMSD bond length	0.010
RMSD bond angle	28.300 *
Data reduction software	SOFTWARE (AT SYNCHROTRON)
Refinement software	X-PLOR (3.851)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	3.200
High resolution limit [Å]	3.000	3.000
Rmerge	0.043 *
Number of reflections	5739
<I/σ(I)>	5.8	3.8
Completeness [%]	98.2	98
Redundancy	4.1	4.0 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, sitting drop *	6.5	24.5 *	pH 6.50

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	0.4 (mM)
2	1	reservoir	sodium cacodylate	50 (mM)
3	1	reservoir		30 (mM)
4	1	reservoir	spermine	1 (mM)
5	1	reservoir		0.9 (M)