1BQU

CYTOKYNE-BINDING REGION OF GP130

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE BM14
Synchrotron site	ESRF
Beamline	BM14
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	1997-02-15
Detector	MARRESEARCH
Spacegroup name	C 2 2 21
Unit cell lengths	84.480, 132.295, 121.933
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 2.000
R-factor	0.221
Rwork	0.221
R-free	0.25500
Structure solution method	MAD
RMSD bond length	0.006
RMSD bond angle	25.300 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Refinement software	X-PLOR (3.851)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.070
High resolution limit [Å]	2.000	2.000
Rmerge	0.058 *	0.206 *
Number of reflections	48189
<I/σ(I)>	19.4	5
Completeness [%]	98.8	96.6
Redundancy	6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	8	4 *	drop consists of equal volume of protein and reservoir solutions *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	12 (mg/ml)
2	1	drop	Tris-HCl	20 (mM)
3	1	reservoir	ammonium sulfate	1.8-2.1 (M)
4	1	reservoir	Tris-HCl	0.1 (M)