1BQM
HIV-1 RT/HBY 097
Experimental procedure
Source type | SYNCHROTRON |
Source details | CHESS BEAMLINE F1 |
Synchrotron site | CHESS |
Beamline | F1 |
Temperature [K] | 108 |
Detector technology | CCD |
Collection date | 1995-03 |
Detector | PRINCETON 2K |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 225.200, 69.400, 104.600 |
Unit cell angles | 90.00, 106.40, 90.00 |
Refinement procedure
Resolution | 10.000 - 3.100 |
R-factor | 0.258 |
Rwork | 0.258 |
R-free | 0.36200 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1tvr |
RMSD bond length | 0.010 |
RMSD bond angle | 25.200 * |
Data reduction software | DENZO |
Data scaling software | CCP4 ((SCALA)) |
Phasing software | X-PLOR (3.85) |
Refinement software | X-PLOR (3.85) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 25.000 | 3.050 |
High resolution limit [Å] | 3.000 | 3.000 |
Rmerge | 0.111 * | 0.890 * |
Number of reflections | 30368 | |
<I/σ(I)> | 11 | 1.1 |
Completeness [%] | 99.1 | 99.5 |
Redundancy | 3.7 | 2.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 6.8 | 4 * | pH 6.8 |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | inhibitor | 20 (mM) | |
10 | 1 | drop | Tris-HCl | 10 (mM) | |
2 | 1 | drop | DMSO | ||
3 | 1 | drop | n-octyl beta-D-glucopyranoside | 20 (%(w/v)) | |
4 | 1 | drop | enzyme | 40 (mg/ml) | |
5 | 1 | drop | 75 (mM) | ||
6 | 1 | reservoir | bis-Tris propane | 50 (mM) | |
7 | 1 | reservoir | ammonium sulfate | 100 (mM) | |
8 | 1 | reservoir | glycerol | 10 (%(v/v)) | |
9 | 1 | reservoir | PEG8000 | 10 (%(w/v)) |