1BQJ
CRYSTAL STRUCTURE OF D(ACCCT)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 103.15 |
| Detector technology | IMAGE PLATE |
| Collection date | 1997-11-01 |
| Detector | RIGAKU RAXIS IIC |
| Spacegroup name | I 2 3 |
| Unit cell lengths | 93.810, 93.810, 93.810 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 15.000 - 2.200 |
| R-factor | 0.192 |
| Rwork | 0.192 |
| R-free | 0.23500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 191d |
| RMSD bond length | 0.004 |
| RMSD bond angle | 23.600 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR (3.851) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 15.000 | 2.300 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.035 | |
| Number of reflections | 7136 | |
| Completeness [%] | 98.5 | 96.3 |
| Redundancy | 4.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 5 (mM) | |
| 2 | 1 | drop | 2-propanol | 5 (%(v/v)) | |
| 3 | 1 | drop | ethanol | 10 (%(v/v)) | |
| 4 | 1 | drop | ammonium sulfate | 0.5 (M) | |
| 5 | 1 | drop | 150 (mM) | ||
| 6 | 1 | drop | 5 (mM) | ||
| 7 | 1 | drop | spermine | 2 (mM) |
