1BQC
BETA-MANNANASE FROM THERMOMONOSPORA FUSCA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | EMBL/DESY, HAMBURG BEAMLINE X11 |
Synchrotron site | EMBL/DESY, HAMBURG |
Beamline | X11 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 1997-03-15 |
Detector | MARRESEARCH |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 43.702, 46.058, 132.511 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 32.000 - 1.500 |
R-factor | 0.119 * |
Rwork | 0.119 |
R-free | 0.17600 |
Structure solution method | MIRAS |
RMSD bond length | 0.007 |
RMSD bond angle | 0.022 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SHARP |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 32.000 | 1.550 |
High resolution limit [Å] | 1.500 | 1.500 |
Rmerge | 0.062 | 0.331 |
Number of reflections | 42667 | |
<I/σ(I)> | 17.2 | 2.9 |
Completeness [%] | 97.2 | 92.2 |
Redundancy | 3.22 | 2.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 7 | 1.85M AMMONIUM SULFATE, 0.1M HEPES, PH 7.0 |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | reservoir | ammonium sulfate | 1.85 (M) | |
2 | 1 | reservoir | HEPES | 0.1 (M) | pH7.0 |