1BPW
BETAINE ALDEHYDE DEHYDROGENASE FROM COD LIVER
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RU200 |
Temperature [K] | 275 |
Detector technology | IMAGE PLATE |
Collection date | 1997-03-15 |
Detector | RIGAKU RAXIS |
Spacegroup name | P 1 |
Unit cell lengths | 85.400, 86.570, 89.810 |
Unit cell angles | 105.32, 113.29, 98.16 |
Refinement procedure
Resolution | 80.000 - 2.800 |
R-factor | 0.239 |
Rwork | 0.239 |
R-free | 0.25700 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1a4s |
RMSD bond length | 0.014 |
RMSD bond angle | 1.700 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 80.000 | 2.980 |
High resolution limit [Å] | 2.800 | 2.800 |
Rmerge | 0.124 * | |
Number of reflections | 49784 | |
<I/σ(I)> | 5 | 1.6 |
Completeness [%] | 92.6 | 88.5 |
Redundancy | 1.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 7.1 * | 14 * | PROTEIN WAS CRYSTALLIZED AT 14 DEGREES FROM 20% PEG 4000, 9.5% ISOPROPANOL, 100 MM HEPES, PH 7.5, temperature 287K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | reservoir | HEPES | 0.1 (M) | |
2 | 1 | reservoir | isopropanol | 9.5 (%) | |
3 | 1 | reservoir | PEG4000 | 20 (%) | |
4 | 1 | drop | protein | 4 (mg/ml) | |
5 | 1 | drop | NAD+ | 1 (mM) | |
6 | 1 | drop | Bis-Tris | 20 (mM) | |
7 | 1 | drop | 150 (mM) | ||
8 | 1 | drop | DTE | 0.1 (mM) |