1BPT

CREVICE-FORMING MUTANTS OF BPTI: CRYSTAL STRUCTURES OF F22A, Y23A, N43G, AND F45A

Experimental procedure

Spacegroup name	P 21 21 21
Unit cell lengths	69.469, 23.346, 29.188
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	? - 2.000
R-factor	0.165 *
Rwork	0.165
RMSD bond length	0.018
RMSD bond angle	0.041 *
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall
Low resolution limit [Å]	10.000 *
High resolution limit [Å]	1.980 *
Rmerge	0.085 *
Number of reflections	2996 *
Completeness [%]	88.0 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	9.7 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein solution	8 (mg/ml)
2	1	drop	potassium phosphate	0.3-0.4 (M)
3	1	reservoir	potassium phosphate	1.5 (M)