1BPO
CLATHRIN HEAVY-CHAIN TERMINAL DOMAIN AND LINKER
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X25 |
Synchrotron site | NSLS |
Beamline | X25 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Detector | MARRESEARCH |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 205.825, 205.825, 87.630 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 6.000 - 2.600 |
R-factor | 0.226 |
Rwork | 0.226 |
R-free | 0.29200 |
Structure solution method | MIRAS |
RMSD bond length | 0.019 |
RMSD bond angle | 2.100 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Refinement software | X-PLOR (3.851) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 100.000 | |
High resolution limit [Å] | 2.600 | |
Rmerge | 0.052 * | 0.261 * |
Number of reflections | 62848 * | |
<I/σ(I)> | 10 | |
Completeness [%] | 96.0 * | 70 * |
Redundancy | 3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 6.5 | pH 6.5 |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | reservoir | PEG4000 | 21-23 (%) | |
2 | 1 | reservoir | 400-600 (mM) | ||
3 | 1 | reservoir | PIPES | 100 (mM) | pH6.5 |
4 | 1 | reservoir | dithiothreitol | 10 (mM) | |
5 | 1 | drop | IPTG | 0.1 (mM) | |
6 | 1 | drop | dithiothreitol | 5 (mM) |