1BPN

DIFFERENTIATION AND IDENTIFICATION OF THE TWO CATALYTIC METAL BINDING SITES IN BOVINE LENS LEUCINE AMINOPEPTIDASE BY X-RAY CRYSTALLOGRAPHY

Experimental procedure

Temperature [K]	123
Spacegroup name	P 63 2 2
Unit cell lengths	129.800, 129.800, 120.700
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	10.000 * - 2.900
Rwork	0.188
RMSD bond length	0.016
RMSD bond angle	3.600
Refinement software	X-PLOR

Data quality characteristics

	Overall
Low resolution limit [Å]	30.000 *
High resolution limit [Å]	2.900 *
Rmerge	0.136 *
Total number of observations	70404 *
Number of reflections	13052 *
Completeness [%]	94.0 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	7.8 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	6 (mg/ml)
2	1	reservoir	MPD
3	1	reservoir	Tris-HCl	50 (mM)
4	1	reservoir		0.05 (M)