1BPM

DIFFERENTIATION AND IDENTIFICATION OF THE TWO CATALYTIC METAL BINDING SITES IN BOVINE LENS LEUCINE AMINOPEPTIDASE BY X-RAY CRYSTALLOGRAPHY

Experimental procedure

Spacegroup name	P 63 2 2
Unit cell lengths	129.100, 129.100, 120.200
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	10.000 * - 2.900
R-factor	0.189 *
Rwork	0.189
RMSD bond length	0.016
RMSD bond angle	3.600
Refinement software	X-PLOR

Data quality characteristics

	Overall
Low resolution limit [Å]	30.000 *
High resolution limit [Å]	2.900 *
Rmerge	0.162 *
Total number of observations	134829 *
Number of reflections	13523 *
Completeness [%]	98.0 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein solution	6 (mg/ml)
2	1	reservoir	MPD
3	1	reservoir	Tris	50 (mM)
4	1	reservoir		0.05 (mM)