1BPH
CONFORMATIONAL CHANGES IN CUBIC INSULIN CRYSTALS IN THE PH RANGE 7-11
Experimental procedure
Spacegroup name | I 21 3 |
Unit cell lengths | 78.900, 78.900, 78.900 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | ? - 2.000 |
R-factor | 0.16 |
RMSD bond length | 0.014 |
RMSD bond angle | 0.043 * |
Refinement software | PROLSQ |
Data quality characteristics
Overall | |
High resolution limit [Å] | 1.900 * |
Rmerge | 0.053 * |
Total number of observations | 21890 * |
Number of reflections | 5760 * |
Completeness [%] | 93.0 * |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Microdialysis * | 9.2 * | referred to 'Dodson, E.J.', (1978) J.Mol.Biol., 125, 387-396 * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | 1 | protein | 20 (mg/ml) | |
2 | 1 | 1 | Na3EDTA | 0.01 (M) | |
3 | 1 | 2 | 0.2 (M) |